机构地区: 中山大学化学与化学工程学院
出 处: 《应用化学》 2004年第1期16-19,共4页
摘 要: 以 β 环糊精为手性添加剂 ,应用胶束电动毛细管色谱电化学检测法对 1 ( 3,5 二羟苯基 ) 2 (甲氨基 )乙醇 (肾上腺素 )进行了手性拆分研究。以金电极为工作电极 ,设定检测电位为 + 0 6 5V(vs SCE) ;考察了环糊精的浓度、SDS的浓度、缓冲溶液pH值、分离电压等实验参数对分离对映体的影响 ,并初步探讨了拆分机理。 A new method for the chiral separation of epinephrine is presented based on the β -cyclodextrin ( β -CD)-sodium dodecyl sulfate(SDS)-micellar electrokinetic chromatography(MEKC) separation of the analytes combined with electrochemical detection using gold microelectrode at +0 65 V( vs SCE). The effects of detection potential, concentration of β -cyclodextrin, concentration of sodium dodecyl sulfate, pH value of electrolyte and loading voltage were discussed. An attempt was made to elucidate the plausible mechanism of the chrial recognition.