作　　者： ; ;

机构地区： 华南师范大学生命科学学院

出　　处： 《生物技术通讯》 2003年第5期393-395,共3页

摘　　要： 采用不同频率的往复机械振荡,以木质藤本植物中华猕猴桃(Actinidiachinensis)茎段的愈伤组织为实验材料,测定ATP含量的变化,并通过与对照组(CK)相应值的比较,研究振荡刺激对植物能量代谢的影响。结果表明,机械振荡对猕猴桃愈伤组织ATP的含量有着比较明显的增强或抑制的双重效应,适度的振荡刺激将有利于提高植物的能量代谢水平,促进植物的生长发育,其中最适的振荡频率为3Hz左右。还从细胞和分子生物学的角度对环境应力影响植物能量代谢的可能机理进行了探讨。 More and more attentions have been taken to the effects of environmental stresses on the growth and de-velopment of plant cells and tissues.Making efforts on this field would enhance plant adaptability to varied environ-ments and to implement extensively efficient agricultural technologies.Using callus cultured from stalks of Actinidia chinensis,this study dealed with the effects of mechanical vibration stimulation on plant cell energy metabolize i.e.content of ATP.The results indicated that there occurs bidirectional effects of ATP content on mechanical vibration.Mechanical vibration frequency of about 3Hz or so are optimal external stresses for the growth and development of A.chinensis.Moderate stresses would be advantageous to A.chinensis.The mechanisms of ATP content effects of me-chanical vibration stimulation on A.chinensis callus was comprehensively discussed in the light of cytobiology and molecular biology.Whereas,the essential mechanisms of biological effects of environmental stresses remains further research.

关 键 词： 中华猕猴桃 愈伤组织 机械振荡

领　　域： [农业科学] [农业科学]