作　　者： ; ; ;

机构地区： 华南师范大学生命科学学院

出　　处： 《水生生物学报》 2003年第6期607-613,共7页

摘　　要： 应用透射电镜对生殖季节与非生殖季节鳜肝脏超微结构的变化进行了观察。鳜肝细胞含有单个卵圆形的核 ,核仁清楚 ;细胞质内含有粗面内质网、线粒体、糖原颗粒和脂滴等细胞器和内含物。胆小管由相邻的数个肝细胞质膜凹陷围成 ,而肝血窦则由内皮细胞的胞质构成。还发现了贮脂细胞、枯否氏细胞和成纤维细胞。胆小管腔和窦周隙内浸润许多由肝细胞发出的微绒毛结构。鳜肝细胞的超微结构在产卵前后呈现明显变化 :产卵前的肝细胞内富含线粒体、糖原颗粒和脂滴 ,粗面内质网发达 ;而产卵后的肝细胞内核仁发生迁移 ,部分细胞核囊泡化 ,糖原颗粒和脂滴排空 ,少数肝细胞具双核结构。非生殖期多数肝细胞核含有双核仁结构 。 The ultrastructure of liver was examined in Siniperca chuatsi Basilewsky at breeding season and non breeding season in their life cycles, with transmission electron microscopy. The hepatocytes contained an oval nucleus. Pronounced nucleoli were observed in each nucleus. Rough endoplasmic reticulum, mitochondria, glycogen and lipid droplets were abundant in cytoplasm of hepatocytes. The bile cannaliculi was makeup by rosettes of up to three hepatocytes. Hepatic sinusoid was formed by endothelial cells.Fat storing cell, Kupffer cell and fibroblasts were also present in the tissue of liver. The lumen of the bile cannaliculi and the space of Disse were occluded by many microvilli extended by hepatocytes. The shape of hepatocytes showed distinct changes before and after spawning, and nucleolus's activity also appeared obvious different. Nucleolar margination in hepatocyte was very rare before spawning, After spawning, nucleolar margination was very obvious and some of nucleolus appeared vesicles; an apparent decrease in the number of glycogen reserves in the hepatocytic cytoplasm, and some of hepatocyte had two nucleus after spawing. In prespawning adults, mitochondria in cytoplasm was abundant as rough endoplasmic reticulum, showing the marked glycogen reserves and the lipid accumulation in the fish. In non breeding season, most of hepatocytes contained two nucleolus, and an apparent increase in the number of lysosome was also found in the hepatocytes.

关 键 词： 鳜 肝脏 生殖季节 超微结构 鲈形目 桂花鱼

领　　域： [农业科学] [农业科学] [生物学]