作　　者： ; ; ; ;

机构地区： 广西民族学院化学化工系

出　　处： 《应用化学》 2003年第12期1152-1156,共5页

摘　　要： 采用未涂层毛细管柱 (5 5cm× 5 0 μm) ,基于H3 BO3 与 β CD络合成大阴离子作为手性拆分试剂 ,在NaOH Cit溶液中加入 9 0mol/LH3 BO3 与 7 0mmol/L β CD(pH =3 0 )作为运行缓冲液 ,用高效毛细管电泳电导检测法对氯霉素前体 (PCP)进行了拆分研究。考察了影响分离的实验参数 ,同时对其拆分机理进行了初步探讨。结果表明 ,在选择的最佳实验条件下 ,氯霉素前体分子中 4个对映异构体在 2 0min内得到基线分离 ,所建立的方法分离条件简单 ,重现性好。 The precursors of chloramphenicol enantiomers were resolved by capillary electrophoresis with conductance detection. The separation was performed on a fused-silica capillary of 50 μm×50 cm. A big anion formed by reaction of H 3BO 3 with β-CD was used as a chiral selector in a NaOH-Cit containing 9.0 mmol/L H 3BO 3 and 7.0 mmol/L β-CD solution(pH3.0). Separation parameters, such as pH, concentration of running buffer and chiral selector, applied voltage were investigated. An attempt was made to elucidate the plausible mechanism of the chiral recognition. It was found that baseline separation of the enantiomers could be accomplished in 20 min under the optimum condition. Results indicated the simplicity and reliability of the enantiotropic separation for the chloramphenicol precursors.

关 键 词： 氯霉素前体 手性拆分 毛细管电泳法

领　　域： [理学] [理学]