作　　者： ;

机构地区： 中国科学院生物物理研究所

出　　处： 《生物物理学报》 1992年第4期585-590,共6页

摘　　要： 酵母GAPDH的剩余活力在碘化钾为0.1M溶液中趋近于零,内源荧光在碘化钾为0.1—0.4M时迅速被淬灭,在0.5—2.0M时基本不变,3.0M时达到最低。并在一定浓度的碘化钾溶液中形成新的荧光发色性质(Ex336nm;Em383nm),量子产率大约为0.2,但形成该荧光发色团较为缓慢。与NAD荧光衍生物(Chen-LuTsou,etal.(1983)Biochem.Soc.Trans.11,425—429)不同的是,GAPDH在碘化钾溶液中形成荧光发色团时不需要光的激发。肌酸激酶及胰岛素不形成这种荧光衍生物。 The intensities of intrinsic fluorescence of yeast D-glyeeraldehyde-3-phos-phate dehydrogenase have been quenched by potassium iodide in three stages; an initial decrease of the intensity of the enzyme in 0.1-0.4 M KI solutions, a little appreciable decrease in 0.5-2.0 M and a decrease to zero over 3.0 M, accompanied with a complete inactivation in less than 0.1 M. Remarkably, a new fluorophore on the enzyme has been slowly formed in the solution around 0.6 M, witk λem 383nm, λex 336nm and a quantum yield about 0.2. On the other hand, CK and insulin do not fot form the fluorophres in the solution. Unlike NAD fluorescent derivatives of GAPDH (Chen-Lu Tsou, et al.: Biochem. Soc. Trans. 11, 1983, 425-429.), this fluorophore is formed withou excitation by xenon lamp,but it appears to be an oxidation procedure of the enny-me with potassium iodide.

关 键 词： 荧光衍生物 碘化钾

领　　域： [生物学]