作　　者： ; ; ; ; ; ;

机构地区： 兰州军区兰州总医院

出　　处： 《西北国防医学杂志》 2003年第5期344-345,共2页

摘　　要： 目的 :了解战士在 5km越野前后的血皮质醇变化。方法 :建立一种直接进样高效液相色谱测定血清中皮质醇的方法。结果 :在波长 2 4 5nm处检测 ,皮质醇的保留时间为 8min ,在浓度 0 .0 5～ 0 .2 5 μg·ml-1范围内显示良好的线性关系 ,最低检测浓度为 4 0ng·ml-1,回收率达 10 0 .89%。越野后战士血清中皮质醇明显升高。结论 :方法简便、准确、专一性强 。 Objective: To observe the changes of serum cortisol level in soldiers after 5- kilometer cross-country training. Methods: A direct injection method for determination of cortisol in serum by RAM-HPLC was developed. Results: The retention time was 8 min and the assay was linear in the range 0.05-0.25 μg.ml -1 monitored at 245 nm. The limit of quantification was 40 ng.ml -1 and the recovery was 100.89%. The cortisol level in soldier serum was raised significantly after cross-country training. Conclusion: The assay was simple, reliable, of specificity and can be served in military stress training.

关 键 词： 皮质醇 浸透限制固定相 高效液相色谱法 应激

领　　域： [生物学]