作　　者： ; ; ; ;

机构地区： 西北农林科技大学食品科学与工程学院

出　　处： 《西北农林科技大学学报（自然科学版）》 2003年第5期111-114,共4页

摘　　要： 　用对比试验和正交试验对甲鱼酶解液化技术进行了研究。结果表明,木瓜蛋白酶的较佳酶解条件为酶用量(E/S)6g/kg,酶解温度65℃,pH值5.5,在此条件下酶解8h后氨基态氮含量为0.262g/kg,木瓜蛋白酶酶解速度慢;胃蛋白酶的较佳酶解条件为酶用量(E/S)42g/kg,酶解温度60℃,pH值1.5,在此条件下酶解6h后氨基态氮含量为0.286g/kg;再将这2种酶在同一底物下酶解,胃蛋白酶酶解速度较木瓜蛋白酶快,6h后酶解液氨基态氮含量为0.1793g/kg,而木瓜蛋白酶8h后酶解液氨基态氮含量为0.1590g/kg;胃蛋白酶酶解甲鱼蛋白液的颜色较木瓜蛋白酶的好;用2种酶复合酶解时,酶解效果较单独使用胃蛋白酶的效果差;酶解液经硅藻土过滤后,酶解液色泽呈乳清色,澄清透亮,适用于制作各种甲鱼保健食品。 This paper is comparative research of enzymatic hydrolysis condition of subtilisin to Trionys sinensis protein.The analysed results of the orthogonal experiment indicate that the optmum conditions for the papain enzymatic hydrolysis were enzyme concentration 6 g/kg,temperature 65 ℃,pH 5.5,taking time 8 h.Then the content of amino nitrogen was 0.262 g/kg.Also,the analysed results of the orthogonal experiment indicate that the optmum conditions for the pepsin enzymatic hydrolysis were enzyme concentratio 42 g/kg,temperature 60 ℃,pH 1.5,taking time 6 h.Then,the content of amino nitrogen was 0.179 3 g/kg.Pepsin enzymatic hydrolysis with better result than papain enzymatic hydrolysis,with pepsin taking time 6 h.The content of amino nitrogen was 0.179 3 g/kg,but the content of amino nitrogen was 0.179 3 g/kg with papain taking 8 h.Enzymes were inactivated at 85 ℃ for 15 min,and the hydrolysate was filtered with diatomite.With bright colour and good taste,the hydrolysate was very good raw material for health food.

关 键 词： 甲鱼 酶解液化技术 对比试验 正交试验 酶用量 酶解温度 值 营养价值 药用价值

领　　域： [轻工技术与工程] [化学工程]