机构地区: 吉林农业大学动物科学技术学院
出 处: 《中国生物工程杂志》 2008年第S1期295-300,共6页
摘 要: 转基因工程菌的构建是基因工程技术中的一个前言课题,它具有良好的产业化前景。其构建包括最佳表达载体的构建,适宜宿主细胞的选择、转化、筛选鉴定和遗传稳定性鉴定,获得质粒能稳定遗传的、高效表达的转基因工程菌等过程。就转基因工程菌及其所用的载体的构建,大肠杆菌、乳酸杆菌、酵母菌和蓝藻菌等受体菌的选育,外源基因的高效表达及其在农业中的饲料用植酸酶基因工程菌,杀虫微生物农药和根瘤菌基因工程菌应用方面进行了概述,并指出了一些转基因工程菌应用的安全性及不够完善的地方。 As a prefatory topic in genetic engineering technology,the construction of transgenic bacteria has the good industry prospect.Construction includes its best expression vector construction,the choice of suitable host cells,transforming,the identification and screening of genetic stability,the genetic stability of the plasmid,and high-level expression of the transgenic bacteria.This article reviews the transgenic engineering bacteria and vector about the construction,the acceptor bacteria such as Escherichia coli,lactic acid bacteria,yeasts and cyanobacteria selection and breeding,exogenous gene expression and application in aspect on agriculture of genetic engineering feed phytase bacteria,pesticides and pesticide Rhizobium microbial genetic engineering,and pointed out the safety problem and the imperfect aspect of transgenic bacteria in the application.
领 域: [农业科学]