作　　者： ; ; ; ; ; ; (陈秋志）; (尹洛毅）; (蒋秋情）;

机构地区： 湖南人文科技学院生命科学系

出　　处： 《食品科学》 2015年第2期52-56,共5页

摘　　要： 采用磷酸缓冲液浸提法提取玉竹糖蛋白粗品(Polygonatum odoratum glycoprotein,PDG),经葡聚糖凝胶G-100和刀豆凝集素A分离纯化,得到糖蛋白组分PDG1和PDG3,经高效液相色谱测定其分子质量,并检测PDG、PDG1和PDG3的体外抗氧化活性。结果表明,PDG1和PDG3的分子质量分别为186 k D和28.3 k D;玉竹糖蛋白具有一定的还原能力,对羟自由基和1,1-二苯基-2-三硝基苯肼自由基具有较强的清除作用,对脂质过氧化具有抑制作用,且在一定质量浓度范围内(1~10 mg/m L)存在剂量关系,其抗氧化能力随着玉竹糖蛋白质量浓度的增加而增强,PDG1和PDG3的抗氧化能力优于PDG。 Crude glycoproteins(PDGs) were obtained from Polygonatum odoratum through phosphate buffer extraction. PDGs were further purified by Sephadex G-100 gel filtration and concanavalin A affinity chromatography, and PDG1 and PDG3 were acquired. The molecular weights and antioxidant activity of these two fractions were determined. The results showed that PDG1 and PDG3 had a molecular weight of 186 and 28.3 k D, respectively, and PDG, PDG1 and PDG3 had reducing power, strong abilities to scavenge hydroxyl radical and 1,1-diphenyl-2-picrylhydrazy(DPPH) radical and inhibitory effects against lipid peroxidation, showing a dose-effect relationship in the range of 1–10 mg/m L. Generally, PDG1 and PDG3 exhibited better antioxidant activity than PDGs.

关 键 词： 玉竹 葡聚糖凝胶 刀豆凝集素 糖蛋白 抗氧化

领　　域： [轻工技术与工程] [轻工技术与工程]