作　　者： ; ; ; ; ;

机构地区： 中国农业科学院茶叶研究所

出　　处： 《食品科学》 2014年第20期164-169,共6页

摘　　要： 建立同时定性和定量分析茶叶中鼠李糖、木糖、阿拉伯糖、果糖、甘露糖、葡萄糖、蔗糖和棉子糖8种单、寡糖的超高效液相色谱-质谱联用方法。样品经超声波辅助提取,以Waters Acquity BEH Amide色谱柱(2.1 mm×150 mm,1.7 μm)分离,以乙腈-水溶液(各含体积分数0.1%氨水)为流动相,电喷雾负离子多反应监测模式检测。结果表明,8种单、寡糖的定量检出限为0.01~0.54 mg/L,线性范围为1.0~200.0 mg/L;添加水平在10.0mg/L和100.0 mg/L内,回收率为80.2%~103.2%。应用该方法分析包含绿茶、乌龙茶、普洱茶和红茶的20个样品,发现大部分样品中都能检测到葡萄糖、果糖、蔗糖和棉子糖,它们的含量分别为0.39~17.64、0.53~22.83、2.07~40.70 g/kg和0.57~14.67 g/kg;但在所有样品中均未检测到鼠李糖、木糖、阿拉伯糖和甘露糖。 An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for the qualitative and quantitative determination of eight saccharides including rhamnose, xylose, arabinose, fructose, mannose, glucose, sucrose, and raffinose in teas. Samples were extracted with the assistance of ultrasound. The extract was separated on a Waters Acquity BEH Aminde HPLC column (2.1 mm × 150 mm, 1.7 μm) using acetonitrile-water mixture (each containing 0.1% ammonia) as the mobile phase. The electrospray ionization tandem quadrupole mass spectrometric analysis was carried out in the negative ion mode using multiple reaction monitoring (MRM). The limits of detection were 0.01–0.54 mg/L and the linear ranges were 1.0–200.0 mg/L. The average recoveries were 80.2%–103.2% at spiked levels of 10.0 and 100.0 mg/L. The method was applied to analyze 20 tea samples including green tea, oolong tea, Pu'er tea and black tea. Glucose, fructose, sucrose and raffinose were detected in most of the samples, and their contents were in the ranges of 0.39–17.64, 0.53–22.83, 2.07–40.70, and 0.57–14.67 g/kg, respectively. However, rhamnose, xylose, arabinose and mannose were not detected in all the samples.

关 键 词： 超高效液相色谱 质谱 茶叶 葡萄糖 果糖 蔗糖 棉子糖

领　　域： [理学] [理学] [农业科学] [轻工技术与工程] [轻工技术与工程]