机构地区: 广西大学轻工与食品工程学院
出 处: 《食品科学》 2014年第19期11-15,共5页
摘 要: 分析过氧化氢诱导罗非鱼皮粗胶原纤维降解过程中,底物的力学性质、超显微结构、红外光谱及X射线衍射谱的变化。结果表明:在过氧化氢存在下,鱼皮胶原纤维的抗穿刺强度随加热时间呈指数下降,其力学变化规律可用指数函数Y=851.Oe^(-0.001x)(R^2=0.996)拟合,拟合性好。扫描电子显微镜说明了鱼皮胶原纤维降解经过纤维束外膜层破坏溶化、纤维束松散断裂、微纤维解旋暴露3个阶段。通过红外光谱和X射线衍射谱检测,说明加热使胶原分子内部作用力减弱,但未溶解物仍保持胶原的三股螺旋结构。 The changes of the mechanical properties,microscopic structure,infrared spectra,and X-ray diffraction spectra of tilapia skin collagen fiber during different stages of its degradation induced by hydrogen peroxide were compared in this paper.The results showed that,in the presence of hydrogen peroxide,the anti-puncture strength of collagen fibers decreased exponentially with heating time.The variations in mechanical properties could be expressed as an exponential function equation Y= 851.0e.0001x(R2=0.996).Scanning electron microscopy demonstrated that the degradation of collage n fiber underwent three stages including melting and destruction of the outer membrane of collagen fiber bundles,loosening and fracture of fiber bundles,and ultimate exposure and release of collagen microfibers.The changes in X-ray diffraction and infrared spectra exhibited weak heating-induced internal force of collagen molecules,but the triple helix was still remained.