作　　者： ; ; ; ; ; ; ; ;

机构地区： 广东海洋大学食品科技学院

出　　处： 《食品与发酵工业》 2014年第5期16-20,共5页

摘　　要： 探讨了几种大表面基质对抗菌脂肽产量的影响,旨在探索抗菌脂肽产量提增的新方法。将凹凸棒、活性炭、纱布等几种大表面积基质加入纳豆菌培养液中培养,通过对特定指示菌的抑菌率计算,测定发酵液中脂肽的生成量,并通过扫描电镜从微观形态上观察菌体在大表面基质上的附着状况,分析大表面基质的作用机制。在相同的发酵条件下,凹凸棒实验组效果最佳,其产生的抗菌脂肽对金葡菌的最高抑菌率为63.2%,较对照组高23.6%,活性炭次之,较对照组高11.9%,纱布组效果较弱,较对照组高9.6%;凹凸棒实验组发酵液中的最大生物量为4.7×108,较对照组高44.7%,活性炭组较对照组高38.5%,纱布组较实验组高38.5%;扫描电镜观察到芽孢杆菌在凹凸棒表面形成的生物被膜组织致密,黏附稳定,附着有高密度的菌体;活性炭表面的生物被膜表现出特殊的褶皱结构,黏附有大量的被膜态菌体,纱布纤维表面形成相对较稀薄但完整连贯的生物被膜并有较多菌体黏附。3种大表面固体基质的加入对抗菌脂肽产量具有明显提增效果,其中凹凸棒的效果最好;产量提升的原因可能与大表面基质的加入增加了发酵液中的菌体浓度以及菌体在大表面固体基质上可以形成生物被膜有关。 This paper concerned the influence of several large surface solid matrixes on the antimicrobial lipopeptide production. It aimed to explore the new method to increase antimicrobial lipopeptide yield. Attapulgite,activated carbon and gauze were added to the main medium of Bacillus natto with some designed amount. The inhibition rate to specific indicator were determined to evaluate the yield of antimicrobial lipopeptide and scanning electron microscopy was used to study the characteristics of bacteria adhered on different solid matrixes and the action mode of large surface matrix. The optimal fermentation was found in the attapulgite experimental group. The inhibition rate of the best experimental group was 63. 2%,which was 23. 6% higher than that of control group,followed by activated carbon group with 11. 9% higher than that of the control group and the gauze group with 9. 6% higher than that of the control group. The results from determination of biomass showed that cell concentration of control group was 44. 7% lower than that of the best attapulgite experimental group,38. 5% lower than that of the activated carbon group and gauze group. Bacillus natto formed stable biofilms on attapulgite with a thick-dense tissue and attached with high density.Bacillus natto formed a special fold structure on activated carbon and attached with large number of biofilm-bacteria.Bacillus natto got relatively thin but integrated biofilms on the surface of gauze fiber and also attached with lots of bacteria. Antimicrobial lipopeptide productivity by Bacillus natto was increased obviously by adding large surface solid matrixes into fermentation medium and addition of attapulgite led to the best productivity. The increased yield may due to the addition of the large surface solid matrixes which increased the biomass in the broth and the biofilms formed on the matrixes.

关 键 词： 纳豆芽孢杆菌 抗菌脂肽 生物被膜 基质

领　　域： [轻工技术与工程] [化学工程]