作　　者： ; ; ; ;

机构地区： 重庆大学生物医学工程联合学院生物流变科学与技术教育部重点实验室

出　　处： 《高技术通讯》 2003年第8期101-107,共7页

摘　　要： 对目前细胞微图型化的概念与适用微加工工艺尤其是软刻技术作了探讨 ,其中包括微压印法、微流体图型化技术与漏印板图型化方法等。进一步 ,从单种细胞的微图型化与不同细胞的微图型化共培养两个方面 ,对许多基底材料 (包括非生物性的与生物性的 )实现细胞微图型化的加工策略 (包括基底的几何形貌与化学修饰剪裁等 ) ,以及这些微图型化基底对细胞贴附与铺展的规约和对细胞后续生存生长等功能的影响等方面的研究进展作了回顾与讨论。 A variety of cell patterning strategies are being developed to microarray and dictate or control the attachment and spreading of each cell on its corresponding location, and establish the lieu of cell microenvironment in vitro. As an emerging technique, it is becoming the supportive one in many other frontier fields, such as the integration of cellular genome and proteome in high-throughput, the deciphering of cellular events during interaction with each other or a surface, and tissue engineering and drug discovery, etc.. Here we review its recent development. For the first, the concept of cell micropatterning and some applicable microfabrication techniques were discussed. And some of soft lithographical techniques for cell patterning, such as microstamping (or μCP), micro-fluid patterning, stencil patterning and others, were briefly introduced. Then, many strategies for patterning cells on surfaces, including non-biologic and biologic, were reviewed, according to one type of cells only and two types of cells common-culture.

关 键 词： 细胞 微图型化 微加工工艺 微接触压印 漏印板图型化 金属基底 光刻胶 自组装单层 非生物性聚合物

领　　域： [生物学]