作　　者： ; ; ; ; ; ;

机构地区： 中山大学生命科学学院水生经济动物研究所

出　　处： 《动物学报》 2003年第4期458-465,共8页

摘　　要： 于 2 2 93± 2 15℃条件下 ,在室外水泥池 (3m× 2m× 1m)中对正常鲈 (2 85 2 6± 6 5 4 g)和患脂肪肝病鲈 (4 6 4 71± 5 4 2 2g)进行为期 9周的饥饿处理。分别在实验开始后第 0周、 3周、 5周、 7周和 9周取样 ,以观察饥饿对于鲈内脏相对重量、肌肉肝脏和血清主要生化指标的影响。研究表明 ,鲈对饥饿耐受能力较强 ,在饥饿时首先快速动用肠系膜脂肪和肌肉脂肪作为能量供应 ,而在整个饥饿阶段则主要以肌肉蛋白质作为能量来源 ,肝脏中能源物质在饥饿中并无明显减少 ,故不是鲈饥饿时的主要供能物质。饥饿时 ,肌肉和肝脏中的水分和脂肪含量呈现负相关 ,尤其在肝脏中表现明显。鲈血清中脂肪酶、甘油三酯、胆固醇、低密度脂蛋白和高密度脂蛋白在饥饿中表现出周期性和阶段性的变化 ,其中正常鲈表现出有规律的波浪状图形 ,而脂肪肝病鲈则表现出山峰状图形 ,说明脂肪肝病鲈代谢机制不如正常鲈灵敏 ,9周的饥饿并不能减轻或消除鲈的脂肪肝病. We investigated the effect of long-time starvation on the use of energy and symptoms of fat liver disease in the Japanese sea bass (Lateolabrax japonicus).Healthy Japanese sea bass(285 26±6 54 g)and Japanese sea bass with fat liver disease (464 71±54 22 g) were maintained in fresh water in outdoor concrete ponds (3 m×2 m×1 m) and starved for 9 weeks. Each treatment was replicated in triplicate. Water temperature in the experimental period was 22 93±2 15℃ and dissolved oxygen, pH and ammonia were 8 17±0 58 mg/L, 7 40±0 45 and 0 54±0 13 mg/L respectively. Samples were collected on weeks 0, 3, 5, 7 and 9 and the effects of starvation on gut weight, the nutritional content of muscle and liver, and some biochemical serum indices were observed. No fish died during the experiment. During starvation, the condition factor, gut ratio and intraperitoneal fat ratio decreased significantly (P<0 05). The hepatosomatic index (HSI) in healthy Japanese sea bass was stable, but in sick Japanese sea bass decreased during the early period(from 1 09±0 14 to 0 65±0 03, P<0 05)after which it remained stable(P>0 05). The moisture content of muscle increased during starvation (healthy Japanese sea bass: from 78 30%±0 28% to 79 72%±0 22%; sick Japanese sea bass: from 76 95%±0 36% to 79 26%±0 11%, P<0 05). The moisture content of the liver of healthy Japanese sea bass decreased from 69 62%±0 59% to 53 10%±2 27% (P<0 05), but remained stable in sick Japanese sea bass(P>0 05). The protein content of muscle significantly decreased during starvation (healthy Japanese sea bass: from 19 63%±0 22% to 18 54%±0 13%; sick Japanese sea bass: from 20 75%±0 15% to 18 90%±0 21%, P<0 05), but fluctuated periodically in the liver(P>0 05). The fat content of muscle decreased during the early period and then remained stable(P>0 05). However, the fat content of the liver increased during starvation (P>0 05)and this change was significant in healthy Japanese sea bass(from 9 84%±0 97

关 键 词： 饥饿 鲈 肌肉 肝脏 血清 生化组成 脂肪肝

领　　域： [生物学]