作　　者： ; ; ; ;

机构地区： 广东省实验动物监测所

出　　处： 《中国病毒学》 2003年第4期401-403,共3页

摘　　要： 犬细小病毒病是由犬细小病毒(Canineparvovirus,CPV)引起的一种多发于幼犬的致死性传染病,主要表现为出血性肠炎和心肌炎[1].在自然条件下本病多呈散发,而在养犬比较集中的地方则常群发[2]. Two pairs of PCR primers were designed according to the sequances of the vaccine strain and virulent strain of CPV. Heminested PCR method was established. Result of the first PCR amplification showed the same amplified products of 574bp length, after the second PCR amplification, the virulent strain produced the length 364bp fragment, but the vaccine strain couldn't produce that. The products of PCR were examined by electrophoresis and restriction enzyme digestion. The result showed the length of the fragment and enzyme sites were as the same as those designed. The PCR assay of CPV was proved to be specific and sensitive. It shows that this method may be used in discriminating the vaccine strain and virulent strain of CPV or monitoring the vaccinated canine in order to aviod disease and financial losing.

关 键 词： 半嵌套式 技术 检测 犬细小病毒 疫苗株 强毒株

领　　域： [农业科学] [农业科学] [农业科学] [生物学]