作　　者： ; ; ; ;

机构地区： 华南农业大学资源环境学院昆虫学系

出　　处： 《华南热带农业大学学报》 2003年第2期1-4,共4页

摘　　要： 利用碱裂解方法抽提广东株家蚕微孢子虫(NosemabombycisNaёgeli,N.b.)基因组DNA,通过PCR技术扩增得到了N.b.的一段特异DNA片段。对该目的片段的序列分析发现,该特异DNA片段大小为317bp,与Malone等报道的日本株N.b.特异DNA片段大小一致,但核苷酸序列同源性仅为92.1%。 The genome DNA of nosema bombycis Nageli(N.b.)was extracted by using SDS and proteinase K.A special DNA fragment was synthesized by PCR amplification.Used as the template,the special DNA fragment was cloned into T Easy Vector and transferred into E.coli DH5α,by which it could be prepared and labeled as a probe to detect N.b.in ser-iculture.The result of sequence analysis of the special DNA fragment showed that the spe-cial DNA fragment was composed of317nucleotides,similar in size to its counterpart in Japanese N.b.,but the homogeneity of Guaangdong N.b.and Japanese N.b.was only92.1%according to this317bp fragment.

关 键 词： 广东株家蚕 微孢子虫 虫害防治 片段 克隆 序列分析 碱裂解方法 核苷酸序列 同源性

领　　域： [农业科学] [农业科学] [生物学]