作　　者： ; ; ; ; ; ; ;

机构地区： 中国农业科学院作物育种栽培研究所农业部作物遗传育种重点开放实验室

出　　处： 《作物学报》 2003年第4期501-507,共7页

摘　　要： 用Xa2 3的近等基因系CBB2 3及其感病轮回亲本JG30构建了包含 2 5 6 2个单株的F2 作图群体。通过分析 5 71个感病单株 ,找到 2个新的与Xa2 3基因连锁的SSR标记RM187和RM2 0 6 ,它们与Xa2 3之间的遗传图距分别为 7 1cM和 1 9cM。通过筛选 12 0 0个RAPD引物 ,获得 2个与Xa2 3基因连锁的RAPD标记RpdH5和RpdS1184 ,与Xa2 3之间的遗传图距分别为 7 0cM和 7 6cM。利用丰富占 /CBB2 3、绿油占 /CBB2 3两个实际育种F2 群体 ,结合抗病性人工接种鉴定 ,测算了RpdS1184、RM2 0 6和RpdH5用于分子标记辅助选择 (MAS)的可能性。结果表明 ,对于丰富 2 3群体 ,RpdH5和RpdS1184的MAS准确率分别为 91 0 %和 87 3% ,如果同时使用这两个标记 ,可使MAS的准确率达 99%。对于绿油 2 3群体 ,RpdH5、RpdS1184和RM2 0 6的MAS准确率分别为 77 1%、81 1%和 80 8%。同时使用RpdH5、RpdS1184标记的MAS准确率为 90 3% ;同时使用RpdH5、RM2 0 6的准确率为 91 3% ;同时使用RpdH5、RM2 0 6、RpdS1184标记的准确率为 90 8%。 A F 2 population, with 2562 individuals, of JG30/CBB23 was constructed for molecular mapping of the bacterial blight resistance gene Xa 23 in rice. After screening 1200 random amplified polymorphic DNA (RAPD) primers, we found RAPD markers, RpdH5 and RpdS1184, were linked to the Xa 23 gene, with genetic distance of 7.0 cM and 7.6 cM, respectively. Two simple sequence repeats (SSR) markers, RM187 and RM 206, were also found to be linked to the Xa 23 gene, the genetic distance were 7.1 cM and 1.9 cM, respectively. The marker assisted selection (MAS) efficiency of RpdS 1184, RM 206 and RpdH5 was evaluated using two true breeding F 2 populations. The results showed that the MAS efficience of RpdH5 and RpdS1184, in population Fengfu23, were 91.0% and 87.3%, respectively, but the MAS efficiency reached as high as 99% when these two markers were co used. For population Luyou 23, the MAS efficiency of RpdH5, RpdS1184 and RM 206 were 77.1%, 81.1% and 80.8%, respectively. When RpdH5 and RpdS1184 were co used, the MAS efficiency was 90.3%; when RpdH5 and RM 206 were co used, the MAS efficiency was 91.3%; when RpdH5, RM 206 and RpdS1184 were co used, the MAS efficiency was 90.8%.

关 键 词： 水稻 抗白叶枯病基因 分子标记 标记定位 辅助选择 人工接种

领　　域： [农业科学]