作　　者： ; ; (王勇）; (张寿洲）;

机构地区： 西安植物园

出　　处： 《园艺学报》 2003年第3期299-302,共4页

摘　　要： 以玉兰亚属的 2 0个材料 (含种和品种 )为研究对象 ,用RAPD技术对其亲缘关系进行分析。从 5 5个随机引物中筛选出 15个 ,对所有供试材料进行扩增 ,共获得 2 74条DNA谱带 ,其中 2 6 2条为多态性带。建立的基于RAPD结果的亲缘关系树形图显示玉兰亚属的基因背景非常复杂 ,类群划分较为困难 ,说明中国的玉兰亚属种质资源非常丰富。 In this study,20 materials of subgenus Yulania(include species and cultivars)were analyzed using random amplified polymorphic DNA markers.15 primers were screened from 55 arbitrary 10-mer primers,and a total of 274 DNA bands were amplified,262 of which(95.6%)were polymorphism.According to the data,a tree diagram of phylogenetic relationship was constructed using UPGMA in MEGA.The results showed that the gene background of subgenus Yulania is very complex and it was difficult to be classified.So know that the resource of subgenus Yulania in China were abundant.

关 键 词： 玉兰 亚属植物 亲缘关系 随机引物 多态性带 种质资源

领　　域： [农业科学] [农业科学]