作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《园艺学报》 2003年第3期272-276,共5页

摘　　要： 选用两对引物组合EcoRⅠACT +MseⅠCTT和EcoRⅠACT +MseⅠCTC对 46份龙眼材料进行AFLP分析 ,共得到扩增位点 111个 ,其中多态性位点 10 3个 ,多态性比例达 92 6 9%,区分率达 10 0 %。对AFLP扩增结果进行聚类分析 ,根据相似系数 0 76的水平可将 46份龙眼品种 (系 )分为 11个品种群。供试各品种多态性比例介于 0 2 70 3和 0 4 95 5间。福建东壁与广东东壁应为不同品系 ;早熟一号为一个新的品系。 The study is mainly on the genetic diversity and relationship of Longan(Dimocarpus longan Lour.) cultivars by applying AFLP(Amplified Fragment Length Polymorphism).103 polymorphic distinct bands were obtained with two pairs of primers(EcoRⅠACT+MseⅠCTT and EcoRⅠACT+MseⅠCTC),which were enough to distinguish the 46 cultivars from each other.UPGMA analysis showed that 46 cultivars were divided into 11 groups.The ratio of polymorphic rate varied from 0.2703 to 0.4955.The result showed that the Fujian Dongbi was different from Guangdong Dongbi and they were different types from Kaiyuansi Dongbi.Zaoshu 1 was supposed to be a new type.The results supplied theoretical basis for cultivar classification.

关 键 词： 龙眼 品种 遗传多样性 亲缘关系 分析 扩增位点 多态性位点 区分率 相似系数

领　　域： [农业科学] [农业科学]