作　　者： ; ; ; ;

机构地区： 仲恺农业技术学院农业与园林学院农学系

出　　处： 《华南农业大学学报》 2003年第3期43-45,共3页

摘　　要： 应用TAC(transformation competitionartificialchromosome)载体和pCAMBIA1300分别与农杆菌LBA4404组合转化水稻成熟胚愈伤组织,探索了农杆菌转化的有关因素.结果表明:共培养培养基上铺上1张滤纸有利于控制农杆菌的过度生长;粳稻品种转化率明显高于籼稻;干燥处理能有效杀死农杆菌,并有利于提高转化率.预再生可提高抗性愈伤组织的再生率而提高转化率. Rice calli from mature embryos were transformed with Agrobacterium LBA4404 harboring plasmid TAC or pCAMBIA1300 , and the factors which influenced transformation were initially studied . The results showed that Agrobacterium wouldn't be overgrown when a piece of paper were placed on coculture media. The transformation rate of Japanica is higher than that of Indica. Agrobacterium could be validly controlled by partial desiccation and the transformation efficiency could be increased greatly. Preregeneration could promote regeneration frequency and transformation efficiency.

关 键 词： 农杆菌介导 水稻 转化条件 成熟胚 愈伤组织 培养基

领　　域： [农业科学]