作　　者： ; ; ;

机构地区： 上海水产大学渔业学院

出　　处： 《上海水产大学学报》 2003年第2期102-105,共4页

摘　　要： 用80个随机引物对黑龙江野鲤(耐寒)、柏氏鲤(不耐寒)和杂交F1(越冬成活)的群体混合DNA样品进行RAPD PCR扩增,结果引物S1043、S1052和S1066扩增出与抗寒性状相关的分子标记各一个。至此,共获得9个与鲤鱼抗寒性状相关的分子标记,进一步表明抗寒性是数量性状(QTL)受微效多基因控制。此外,就RAPD PCR实验中需要注意的问题如模板纯度、水的去离子和不同商标酶的活性等进行了简单的讨论;并对目前所应用的淡水鱼类抗寒基因工程研究策略及现状进行了简单概述。 Population blending DNA samples respectively derived from Heilongjiang wild carp Cyprinus carpio haematopterus Temmink et Schlegel(cold tolerance), Boshi carp Cyprinus pellegrini pellegrini Tchang(cold sensible) and F1 generation(survivors experienced through winter), were programmed RAPDPCR with 80 random primers. Primer S1043, S1052, S1066 resulted in one molecular marker respectively. Up to now, 9 markers associated with cold tolerance of common carp were obtained. It further demonstrates that cold tolerant trait is a quantitative trait loci(QTL), which is controlled by small multigenes. In addition, author simply discussed the experimental methods, such as template purity, water deionization and enzyme activity with different brands. Author also sketched study tactics and present conditions on cold tolerant gene engineering for fresh water fishes.

关 键 词： 随机扩增多态性 聚合酶链式反应 抗寒基因工程 抗冻蛋白 基因 性状 鲤鱼

领　　域： [生物学] [农业科学]