作　　者： ; ; ; ; ; ;

机构地区： 中国农业大学生物学院

出　　处： 《中国实验动物学报》 2003年第2期88-91,共4页

摘　　要： 目的　探讨一氧化氮供体—硝普纳 (SodiumNitroprusside ,SNP)对昆明小鼠卵母细胞体外自发成熟的影响。方法　利用体外培养方法 ,在培养的不同时间观察卵母细胞的成熟情况 ,研究SNP对卵母细胞自发成熟的动力学影响。结果　 (1) 1mmol LSNP能够明显延迟卵丘卵母细胞复合体 (CEOs)的自发成熟 ,与对照组相比 ,CEOs的生发泡破裂 (GVBD)和第一极体 (PB1)释放分别延迟了 8h和 10h。但在培养结束 (2 4h)时 ,CEOs处理组的PB1释放率与对照组相比有明显的降低 ,而处理组的生发泡破裂 (GVBD)百分率和对照组相比没有明显变化 ;1mmol LSNP能够明显延迟裸卵母细胞 (DOs)的自发成熟 ,与对照组相比 ,DOs处理组的GVBD和PB1释放分别延迟了 6h和 12h。且在培养 2 4h后 ,DOs处理组的GVBD和PB1释放率与对照组相比有显著的降低。 (2 ) 1mmol LSNP能够明显促进CEOs中卵丘细胞的离散 ,造成CEOs互相粘连在一起。 (3) 1mmol LSNP能够明显影响体外培养的DOs的形态 ,但对CEOs的卵母细胞却没有影响。而 1μmol LSNP对CEOs和DOs的自发成熟都没有影响。 Objective To investigate the effect of Sodium Nitroprusside(SNP),an NO donor,on the oocyte spontaneous maturation of Kunming white mouse in vitro. Methods Oocytes were cutured in vitro and examined at different stage,and the effect of SNP on the kinetics of spontaneous maturation of mouse oocyte was valued.Results\ (1)1 mmol/L SNP significantly postponed the spontaneous oocyte maturation of CEOs.Compared with control,GVBD and PB1 extrusion were put off 8 and 10 hours,respectively.But at the end of culture (24h),the percentage of GVBD had no obvious difference compared with control,whereas the percentage of PB1 of the treated group was still significantly lower than that of control.Moreover,1 mmol/L SNP had a significant suppression on the spontaneous maturation of DOs.GVBD and PB1 were put off 6 and 12 hours compared with control,respectively.And at the end of culture,the percentages of GVBD and PB1 of the treated group both decreased significantly compared with control.(2)1 mmol/L SNP significantly stimulated the dispersal of cumulus cells from their oocytes,which made CEOs conglutinate together.(3)1 mmol/L SNP could significantly affect the morphology of DOs,but had no influence on CEOs.We also studied the effect of 1μ mol/L SNP and the result demonstrated that 1 μ mol/L SNP had no any effect on spontaneous oocyte maturation of CEOs and DOs.Conclusion\ A suppressive effect of high concentration of SNP was produced on the spontaneous maturation of mouse oocytes in vitro.

关 键 词： 一氧化氮 硝普纳 小鼠 卵母细胞 体外自发成熟 信息分子

领　　域： [生物学] [生物学]