作　　者： ; ; (胡建东）; (张晓梅）; (王建波）; (王宝山）;

机构地区： 河南农业大学机电工程学院

出　　处： 《农业工程学报》 2015年第S2期174-179,共6页

摘　　要： 传统的叶绿素无损检测方法采用是的光学透射法,它的测量值是一个相对值。植物叶片的厚度显著的影响了测量的准确性。该文利用三波长漫反射的技术设计了一个试验装置。选取了3种玉米品种,郑单-958,浚单-20和豫单-606来验证检测装置的可行性。在玉米叶片漫反射函数值和叶片叶绿素真实值之间建立了回归分析,郑单-958,浚单-20和豫单-606的决定系数R2分别为0.9766,0.9612和0.9409。试验结果证明了三波长漫反射光谱的方法测量玉米整体叶绿素含量的可行性。 Transmission colorimetric method is a traditional method of chlorophyll content nondestructive determination. Plant leaves thickness significantly affect the accuracy of measurement. In this paper, an experimental instrument using a three-wavelength diffuse reflectance was proposed. Three different maize breeds, Zhengdan-958, Xundan-20 and Yudan-606 were verified by using this instrument. The regression analyses between remission function values of diffuse reflectance and the real content of total chlorophyll were established. The determination coefficients of R2 between remission function values and the real content of total chlorophyll were 0.9766 for the Zhengdan-958 leaves, 0.9612 for the Xundan-20 leaves and 0.9409 for the Yudan-606 leaves. The experimental results showed the feasibility of diffuse reflectance spectrum for the detection of the total content chlorophyll.

关 键 词： 无损检测 叶绿素 光谱分析 三波长 玉米 漫反射

领　　域： [农业科学]