机构地区: 首都师范大学生物系
出 处: 《首都师范大学学报(自然科学版)》 2003年第2期64-67,共4页
摘 要: 将克隆在不同质粒上的thaumatin基因的两个片段连接起来 ,连接产物克隆到质粒pUC18上 .测序结果表明 ,连接产物是一个完整的thaumatincDNA基因 .将此基因通过基因重组技术 ,克隆到植物表达载体pBI12 1中 ,构建了一个新的转化植物的表达载体—pBI12 1 tha. After cheated with T\-4 ligase, the two fragments which were cloned into two different plasmids were ligated into a complete thaumatin gene. The traget gene was confirmed by sequencing. By DNA recombinatnt methods, the cDNA gene was cloned into vector pBI121.A new expressing vector\_\_pBI121\|tha was successfully constructed.