作　　者： ; ;

机构地区： 西北农业大学植物保护系

出　　处： 《华南农业大学学报》 1992年第2期5-11,共7页

摘　　要： 本文报道了川檩素对菜青虫幼虫呼吸作用的影响及其它几种生理指标的测定结果,川楝素中毒的幼虫呼吸强度降低,呼吸商升高,呼吸节律完全失去控制而为直线型。结合幼虫血淋巴总量、比重、含糖量、pH值、蛋白含量及肠液pH值等生理指标测定结果,说明川楝素中毒的菜青虫幼虫的生理代谢受到干扰,体内进行不正常的生物氧化,且代谢水平降低.同时也证明对幼虫中枢神经有抑制作用。 Determinations of respiration and other physiological parameters of imported cabbage worm (Pieris ropae L.) treated with toosendanin,an extract from the bark of Melia toosendan,were conducted in the Laboratory of Insect Toxicology,South China Agricultural University in 1988.In the experiments on respiration with a Warburg respirameter,marked decrease in oxygen uptake was observed.It was 0.137 1 ml·g-1·hr-1 in the controls as compared with 0.094 ml·g-1·hr-1 in the treated larvae.The respiratory quotient increased from 0.841 7 (control) to 0.970 3 (treated ) and even up to 1.042 6 (paralysed larvae).The respiratory rhythm as examined with Infra-red CO2 Analyzer was interfered with greatly,no respiratory rhythm was observed in the intoxicated and paralysed larvae.Determinations of some physiological parameters showed that total volume of hemolymph in the treated larvae did not change significantly,but the pH of the hemolymph increased from 6.78 (control) to 8.16 (48 hr after treatment),and the pH of intestinal juice decreased from 9.17 (control) to 7.82 (72 hr after treatment).The protein content in the hemolymph and in the tissue of the ventriculus of the treated larvae also decreased markedly.The larvae after starving for 3 days did not show any change in pH in the hemolymph and intestinal juke,but the volume of hemolymph decreased significantly,being 38.4%of the control.This seemed to indicate that the pathological changes in the larvae treated with toosendanin were not caused by its antifeedant properties.All the results of the observations indicated that the metabolism of the treated larvae was interfered with and the normal activities of the nervous system were also inhibited by toosendanin.

关 键 词： 川楝素 菜青虫 植物质 杀虫剂 害虫

领　　域： [农业科学] [农业科学]