作　　者： ; ; ;

机构地区： 华中农业大学动物科学技术学院农业部猪遗传育种重点开放实验室

出　　处： 《华中农业大学学报》 2003年第2期110-113,共4页

摘　　要： 以大白等 6个猪品种为试验材料 ,建立了家猪扩增酶切片段长度多态性 (AFLP)分析体系 ,对其分析过程中DNA提取、双酶切、连接、预扩增、选择性扩增、银染效果进行了检测。用E39M 4 8引物组合构建了供试猪的AFLP指纹图谱。条带清晰可辨 ,扩增信号强 ,无背景干扰。 AFLP (Amplified Fragment Length Polymorphism) analysis system was established with four pig species. The results of several reactions during AFLP analysis have been detected such as DNA extraction, double enzymes restriction, adadpter ligation, preamplification and selective amplification .The AFLP fingerprinting of four pig species with primer combination E39M48 have been performed, which have 70 clear and strong amplification bands with bright background.

关 键 词： 家猪 分析体系 提取 双酶切 连接 预扩增 选择性扩增 指纹图谱

领　　域： [农业科学] [农业科学]