作　　者： ; ; ; ; ; ;

机构地区： 同济大学医学院

出　　处： 《同济大学学报（医学版）》 2003年第2期100-103,共4页

摘　　要： 目的　探讨β 胡萝卜素对氧化镍致人肺成纤维细胞DNA损伤作用的影响。方法　应用单细胞凝胶电泳技术 (singlecellgelelectrophoresis,SCGE)或称彗星电泳技术 (cometassay)比较研究。 结果　氧化镍 (Ni2 O3 )可引起人肺成纤维细胞DNA双链断裂损伤 ,Ni2 O3 与β 胡萝卜素共同处理的人肺成纤维细胞的DNA双链损伤较之Ni2 O3 单独处理组降低 (P <0 .0 1)。结论　氧化镍可引起人肺成纤维细胞DNA双链损伤。β 胡萝卜素可明显降低Ni2 O3 对人肺成纤维细胞DNA链的损伤作用。SCGE是一种简便、快速、敏感及经济的细胞基因毒性检测方法 ,可应用于各级水平的环境与人类疾病 (镍污染 )检测与研究。 Objective To study the influence of β-carotene on Ni 2O 3-induced DNA strand breakage of human lung fibroblast cells and to explore the application of single cell gel electrophoresis(SCGE) in detecting genotoxicity.Methods Single cell gel electrophoresis(SCGE) assay or comet assay was used to study the effects of β-carotene on Ni 2O 3-induced DNA strand breakage of human lung fibroblast cells.Results Ni 2O 3 could induce DNA double strand breakage in human lung fibroblasts cells as compared with the control group(P<0.01),and the damage ratio clould descend singnificantly by using β-carotene and Ni 2O 3 in human lung fibroblast cells as compared with that of the Ni 2O 3 group(P<0.01).Conclusion The doublestrand DNA breakage of human lung fibroblast cells can be induced by Ni 2O 3.β-carotene can degrade observably the DNA double strand breakage induced by Ni 2O 3.SCGE assay is a simple,convenient,rapid,sensitive and economic method in detecting genotoxicity.It can be applied to all levels of monitoring and studing environmental pollution and human diseases(Nickel pollution).

关 键 词： 胡萝卜素 氧化镍 人肺成纤维细胞 损伤 作用 影响

领　　域： [生物学]