作　　者： ; ; ; ;

机构地区： 广州军区广州总医院

出　　处： 《解放军医学杂志》 2003年第4期319-320,共2页

摘　　要： 为了探索DNA传感器用于DNA定量定性分析的可行性 ,研制了石英晶体DNA传感器 ,并对其性能进行了研究。用生物素法将单链DNA探针固定在镀金石英晶体表面上制成DNA传感器 ,再用该传感器检测溶液中的互补单链DNA和单碱基错配的互补单链DNA ,并用 0 1mol/LHCl对杂交后的传感器进行了再生。结果显示 ,10MHz石英晶体DNA传感器的杂交灵敏度为 10ng/ml,在 5～ 4 0ng/ml的浓度范围内 ,响应信号与检测浓度之间具有良好的线性相关性 ,其特异性也比较高。杂交后的DNA传感器经再生后可反复利用 5次以上。 In order to investigate the possibility of analysis of single strand DNA by using DNA biosensor, we developed a quartz crystal DNA biosensor and studied its performances. The single strand DNA probe was immobilized on the surface of gold filled quartz crystal by biotin immunoreaction. The DNA biosensors were used to detect the complete complementary and single base mismatch complementary single strand DNA in solution. The DNA biosensors were regenerated with 0 1mol/L HCl after hybridization. The hybridization sensitivity of quartz crystal DNA biosensor is 10ng/ml. In the range of 5～40ng/ml, there was a linear correlation between responding signals and concentrations of complementary single strand DNA with a high specificity. The DNA biosensor could be repeatedly used over 5 times by using 0 1mol/L HCl for regeneration.

关 键 词： 生物素 免疫膜 石英晶体 传感器 研制

领　　域： [生物学]