作　　者： ; ; ;

机构地区： 浙江大学生命科学学院生物技术系

出　　处： 《浙江大学学报（工学版）》 2003年第1期121-123,共3页

摘　　要： 为了建立螺旋藻外源基因表达体系,首先必须选择螺旋藻抗性基因.本实验选用三种抗生素进行敏感性研究,实验结果表明:螺旋藻对这三种抗生素的敏感性有明显差异,它们对螺旋藻生长的抑制作用依次为:氯霉素>链霉素>氨苄青霉素.氯霉素对螺旋藻生长的抑制作用与氯霉素的浓度和作用时间呈正相关.600mg/L氯霉素处理48h就对螺旋藻有明显的致死作用,致死率分别为:48h为17.51%、144h为68.67%;400mg/L的氯霉素:48h至死率为1.96%、144h为49.76%;200mg/L以下低浓度的氯霉素对螺旋藻的抑制作用需要较长时间,144h才具有明显的致死作用,致死率为19.24%. In the work, three different antibiotics were tested to determine Spirulina platensis' special sensitivity as a foundation of selecting the resistant gene and constituting Spirulina's expression system for recombinant foreign gene. The results revealed that the inhibition reaction to growth of Spirulina platensis was in the order Chloromycetin (Cm) > Streptomycin sulfate> Ampicillin. The inhibition of Cm on Spirulina platensis growth enhanced with increase both of Cm concentration and lapse of time. When the concentration of Cm was 600 mg/L, the death rate of Spirulina was 17.51% at 48 h?68.67% at 144 h;When the concentration of Cm was 400 mg/L, the death rate was 1.96% at 48 h?49.76% at 144 h; The death rate was 19.24% at 144 h when Cm concentration was as low as 200 mg/L. 

关 键 词： 钝顶螺旋藻 基因工程 抗生素 生长抑制作用 抑制效应 抗性基因 光合表达系统

领　　域： [生物学] [生物学]