机构地区: 中山大学生命科学学院
出 处: 《中山大学学报(自然科学版)》 2003年第2期70-73,共4页
摘 要: 采用内生菌分离法对华南种植的健康香蕉根、叶内生真菌、内生放线菌进行分离研究表明:叶内部的内生真菌主要为盘长孢刺盘孢菌Colletotrichum gloeosporioides占60.7%;而根内部曲霉Aspergillus spp.,青霉Penicil-lium spp.,拟青霉Paecilomyces spp.的分离频率较高,共占70.4%。在根、叶内生放线菌中灰红紫类群链霉菌分离频率最高,分别占65.2%与78.2%;在整个植株中玫瑰浅灰链霉菌Streptomyces roseogriseolus的分离频率最高(占57.7%),叶中该种链霉菌占69.0%,根中占43.5%。对峙实验表明:内生真菌在体外对香蕉枯萎病的病原菌(尖孢镰刀菌香蕉专化型)不显示明显的拮抗作用而内生放线菌则显示明显的拮抗活性,玫瑰浅灰链霉菌中有42.5%的菌株显示拮抗作用,该种链霉菌在提高香蕉植株对香蕉枯萎病的抗性中的作用值得重视。 Two hundred and seventy one endophytic fungal strains and one hundred and fifty-six endophytic actinomycete strains were isolated from healthy banana plants. The fungi isolated most frequently from leaves were Colletotrichum gloeosporioides (60.7%) and fungi from roots were Aspergillus spp., Penicillium spp. and Paeciloniyces spp. (total 70.4%). Streptomyces strains were the most frequently isolated actinomycete strains from roots and leaves, gray series of streptomycete strains were the most frequently isolated streptomycete group, Streptomyces roseogriseolus were the most frequently isolated species from roots (43.5%) and leaves (69.0%) and 46.7% of Streptomyces roseogriseolus strains isolated from roots and 34.8% of strains from leaves showed activity against Fusarium oxysporum f.sp. cubense. But endophytic fungi isolated were not antagonistic to Fusarium oxysporum f. sp. cubense in vitro.