作　　者： ; ; ; ; ;

机构地区： 中山大学

出　　处： 《中山大学学报（自然科学版）》 2003年第2期66-69,共4页

摘　　要： 应用SMARTTM技术构建了斜带石斑鱼Epinephelus coioides卵巢cDNA文库.所构建的cDNA文库滴度为4.5×106pfu/mL,重组率为99%,扩增后文库的滴度为1.2×1010 pfu/mL.把phagemid转化为质粒后,随机挑选60个阳性克隆进行酶切鉴定和测序.酶切结果表明:插入片段长度均在500～3 000bp之间.测序结果经过生物信息学分析,发现其中一个克隆为脂肪酸结合蛋白(FABP)基因全长,并与人肝型(L)脂肪酸结合蛋白同源性最高,为70%. A cDNA library from the ovary of Epinephelus coioides was constructed using the SMART?technique. The liter of the primary library was 4.5 × 106 pfu/mL; the recombination rate was 99% ; and the titer of the amplified library was 1.2 ×1010 pfu/mL. After conversion of phagemid to plasmid, the positive clones were selected randomly and sequenced, and results showed that the sizes of the inserts were from 500 bp to 3 kb. After bioinformatic analysis, a clone of fatty acid binding protein gene was obtained, and it shared highest homology (70%) with human liver-type (L) fatty acid binding protein gene (L-FABP) .

关 键 词： 斜带石斑鱼 卵巢 文库 脂肪酸结合蛋白 基因克隆 鲈形目 酶切鉴定

领　　域： [生物学] [生物学]