作　　者： ; ; ; ;

机构地区： 华南农业大学理学院

出　　处： 《华南农业大学学报》 2003年第2期51-53,共3页

摘　　要： 采用富含自由氨基的丝素 壳聚糖合金膜为载体,吸附固定从柞蚕蛹提取分离的超氧化物歧化酶(SOD),研究并确定了固定化的最佳条件,分别为酶浓度38U/mL、pH6 3、温度4～8℃、时间15h.制得的固定化酶活力为89 1U/g载体,酶的活力回收达到35 9%. Taking fibroinchitosan blend membranes that contained abundant amino groups as carrier, superoxide dismutase(SOD) extracted from the Tussah pupa was immobilized.The optimal conditions for immobilization of the enzyme were obtained. The optimal conditions were enzyme concentration 38 U/mL, pH 63,temperature 4～8℃ and time 15 h, respectively.The activity of immobilized enzyme was 891 U/g, and enzymatic activity recovery reached 359%.

关 键 词： 丝素 壳聚糖合金膜 超氧化物歧化酶 固定化 柞蚕蛹 酶活力

领　　域： [生物学] [生物学]