作　　者： ; ; ; ;

机构地区： 暨南大学生命科学技术学院化学系

出　　处： 《分析测试学报》 2003年第2期75-77,共3页

摘　　要： 在固定电压下 ,将单链DNA固定在活化的碳糊电极 (CPE)表面 ,以电活性物质Co(bpy)3(ClO4)3 为指示剂 (bpy为联吡啶 ) ,研究了单链DNA电极 (ssDNA_CPE)、双链DNA电极(dsDNA_CPE)的指示剂还原峰电流变化 ;研究了在一定电压下 ,杂交时间对指示剂的电化学信号的影响,实现了用DNA电化学传感器快速检测人类免疫缺陷病毒 (HIV)基因的初步探索。 ssDNA was fixed on the surface of carbon paste electrode(CPE)which had been activated in certain potential.Co(bpy) 3 (ClO 4 ) 3 (bpy is bipyridine)was used as an electroactive indicator.The reduction peak current of the indicator on ssDNA-CPE and dsDNA-CPE were observed and compared.The effect of hybridization time and insertion time of the indicator on the eletrochemical signal of the indicator in certain potential was examined.Fast diagnosis of human immunodeficiency virus gene was preliminarily probed using the developed DNA electroˉchemical sensor.

关 键 词： 电化学传感器

领　　域： [理学] [理学] [生物学]