作　　者： ; ; ; ; ; ;

机构地区： 沈阳农业大学畜牧兽医学院

出　　处： 《畜牧兽医学报》 2003年第2期147-151,共5页

摘　　要： 给体重约 5 0kg的长白×太湖仔猪以 0 2 5、0 5、1 0、2 0mg剂量半腱肌部位注射 pGRF基因的表达质粒 ,5～ 10kg阶段终体重分别比 0mg组增加 4%、17%、2 0 %和 16 % ,日增重增加 8%、37%、43%和 36 % ,采食量提高 17%、2 7%、2 3 %和 11% ;耗料增重比 ,0 5mg组、1 0mg组及 2 0mg组分别比 0mg组降低 8%、14%和 18%。1 0mgpGRF基因质粒使血液中生长激素释放因子 (GRF)比对照组升高 1 6倍 (P <0 0 5 )、生长激素 (GH)升高2 5倍 (P <0 0 1)、类胰岛素生长因子 (IGF Ⅰ )升高 2 3倍 (P <0 0 1) ,血液中尿素浓度降低 2 0 % (P <0 0 5 ) ,血液中甘油三酯浓度 (TG)明显下降 (P <0 0 5 )。试验研究还证明生长抑素 (SS)分泌的增加是限制 pGRF基因质粒高用量时正效应发挥的重要因素 ,1mg为 5～ 10kg阶段仔猪注射 pGRF基因质粒的适宜剂量。 Landrace×Taihu piglets with an initial weight of about 5 kg were selected and divided into 5 groups randomly.Each group was alloted to one of five treatment,injected with 0?0.25?0.5?1.0 and 2.0 mg pGRF gene plasmid in semitendinous muscle and raised till the body weight reached about 10 kg.The result showed that:injecting of pGRF gene plasmid increased the end weight by 4%?17%?20% and 16%,the average daily gain by 8%?37%?43% and 36%,the average daily intake by 17%?27%?23% and 11%.0.5?1.0 and 2.0 mg pGRF gene plasmid made the feed-consumption/gain dropped by 8%?14%?and 18%.The GRF concentration in 1.0 mg group is 1.6 times(P<0.05),GH is 2.5times(P<0.01)and IGF-Ⅰ is 2.3 times(P<0.01)higher than the control.Treatment of 1.0 mg pGRF gene plasmid made the blood urea (BU)concentration and triglyceride(TG)concentration dropped down dramatically(P<0.05).The increasing of SS(somatostatin)secretion after the injection is the limiting factor for the use of pGRF gene plasmid to improve animal production potential.1.0 mg is the proper dose for piglets when injecting pGRF gene plasmid.

关 键 词： 基因质粒 仔猪 骨骼肌 代谢调控

领　　域： [生物学]