作　　者： ; ; ; ; (马文丽）;

机构地区： 华南理工大学食品与生物工程学院

出　　处： 《昆明医学院学报》 2003年第1期13-16,共4页

摘　　要： 利用通用载体质粒融合系统UPS (univectorplasmid -fusionsystem)构建出了以GFP (绿色荧光蛋白 )为报告基因的酵母表达载体 ,经PCR、电泳、测序等手段证明了构建的准确性 。 S. cerecisiae expression vector was constructed by UPLS(univector plasmid-fusion system) with GFP as report gene. By the way of PCR, electrophoresis and sequence measurement and so on, the result showed accuracy of construction, and simultaneously, high efficiency and convenience of UPS were also testified.

关 键 词： 酵母表达载体

领　　域： [生物学]