作　　者： ; ; ; ; ;

机构地区： 吉林农业大学动物科学技术学院

出　　处： 《吉林农业大学学报》 2002年第6期68-71,共4页

摘　　要： 在克隆AcrA、AcrB部分基因片段并进行基因及编码蛋白的同源性分析的基础上,设计合成构建AcrA、AcrB的内标准DNA所需引物,经过3次(12个)PCR成功地合成了AcrA、AcrB的内标准DNA。合成法构建定量RT-PCR内标准DNA快捷、成功率高。 Based on the cloning of their parts and the analysis of homology of their genetic nucleotide sequence and deduced amino acid sequence, primers of internal standard DNA of gene AcrA. and AcrE were designed and synthesized. The internal standard of AcrA. and AcrE was synthesized by 12 ploymerase chain reactions. Construction of internal standard of quantitative RT - PCR with synthesis method was quickly and easily accomplished.

关 键 词： 大肠杆菌 耐药基因 内标准 合成法 引物 动物

领　　域： [农业科学] [农业科学] [农业科学]