作　　者： ; ;

机构地区： 云南省森林灾害预警与控制重点实验室

出　　处： 《华中农业大学学报》 2016年第4期42-48,共7页

摘　　要： 为明确黄单胞菌Xanthomonas campestris pv.raphani 756C(Xcr)中存在的Ⅵ型分泌蛋白(Tss)数量及其所具有的信号肽、保守motif等信息以及该菌中Tss与其他病菌中同源序列之间的遗传关系,利用关键词对Xcr蛋白质数据库进行搜索,并对Xcr中Tss氨基酸序列开展信号肽、跨膜结构域以及保守基序(motif)的生物信息学分析,同时,对Xcr中所含有的Tss与其他病原菌中同源序列之间的遗传关系进行分析。明确Xcr中存在3个Tss,分别命名为TssA、TssB、TssC,上述Tss均含有高于50%比例的!螺旋结构,均定位在细胞膜上以及具有3个保守motif,而就信号肽而言,仅TssC含有明显的信号肽序列。Xcr中的Tss与Xcc、Xca等黄单胞菌属病菌中的Tss具有较近的亲缘关系。 To determine the types,signal peptide,and conserved motif of the typeⅥ secretion proteins(Tss)in Xanthomonas campestris pv.raphani 756C(Xcr),and to provide an important theoretical guidance for clarifying the Tss of the other Xanthomonas pathogen,Tss sequence of Xcr from the protein databases was searched using keywords,and the conserved domain was searched using SMART online.The signal peptide,transmembrane domain structure,the tertiary structure and conserved motif were analyzed.Meanwhile,the genetic relationships was analyzed using comparative Tss in Xcr and homologous sequences of other species.There are three Tss in Xcr,namely TssA,TssB,TssC,which have more than 50% helical secondary structure and only TssC contains the typical signal peptide sequence.All the 3Tss positioned in the plasma membrane,and each has 3conserved motifs.Based on the genetic analysis of Tss,it is suggested that Xcc and Xca are closely related to Xcr.

关 键 词： 型分泌系统 型分泌蛋白 黄单胞菌属

领　　域： [农业科学] [农业科学] [农业科学]