机构地区: 南车株洲电力机车研究所有限公司
出 处: 《光谱学与光谱分析》 2002年第6期1051-1053,共3页
摘 要: 本文基于脱氧核糖核酸 (DNA)对有机染料副品红的共振光散射的增强效应 ,拟定了一种测定DNA的共振光散射法。在pH =0 5~ 1 5范围内 ,有机染料副品红于 35 5nm处的共振光散射强度被DNA强烈增强 ,且增强程度与DNA浓度呈线性关系 ,线性范围为 0 10~ 15 μg·mL- 1 ,检出限可达 36ng·mL- 1 。该方法简便、快速、具有较高的灵敏度和准确度 ,且线性范围较宽。将该方法用于混合样品中DNA的测定 ,结果令人满意。 The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to. the concentration of DNA in the rang of 0.20-15 mug.mL(-1). The detection limit is 36 ng.mL(-1). This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples.