作　　者： ; ; ;

机构地区： 浙江大学药学院

出　　处： 《浙江大学学报（医学版）》 2002年第6期433-436,460,共5页

摘　　要： 目的 :研究一种新型的工艺简单的适合工业化生产的干扰素 -α脂质体制备方法。方法 :采用均匀设计法筛选最佳处方 ,粉末床研磨法制备空白前体脂质体 ,与干扰素混合得到干扰素 -α脂质体 ,库尔特粒子分析仪测定粒子大小和分布 ,凝胶过滤法分离纯化脂质体 ,酶联免疫方法 (EL ISA)测定其包封率。结果 :干扰素 -α脂质体制备的最佳条件 :保护剂为山梨醇 ,保护剂与脂质的质量比为 5∶ 1,十八胺和脂质的质量比为 1∶ 9,磷脂与胆固醇质量比为 9∶ 1。制备所得的干扰素 -α脂质体的粒径大小为 (80 .8± 36 ) nm ,包封率为 (5 9.0± 3.3) %。结论 Objective: To investigate a nwe, simple technique for preparation of interferon α liposomes, which may be suitable for industrial use. Methods:The uniform design coupled with computerized optimization was utilized to screen the formulation and preparation procedure of interferon α liposomes. Pro liposomes were prepared by the powder bed grinding method and combined with interferon α solution to form interferon α liposomes. Liposome size was determined by the particle size analyzer. Free interferon α and interferon α liposome were separated by gel filtration. Then the recovered activity of interferon α was analyzed by enzyme linked immunosorbent assay. Results:The result demonstrated that the best interferon α liposome formulation was as follows: the protectant was sorbitol; weight ratio of protectant to lipid was 5∶1; weight ratio of octadecytamin to lipid was 1∶9; weight ratio of sobey phosphatidylcholine to cholesterol was 9∶1 respectively. Interferon α liposome size determined by the particle size analyzer was 80.8±36 nm and the encapsulation efficiency was 59.0±3.3%. Conclusion:The powder bed grinding method can be used to prepare pro liposomes which can be easily combined with interferon α solution to form interferon α liposomes.

关 键 词： 干扰素 化学 粉末床研磨法 前体脂质体 均匀设计

领　　域： [化学工程]