作　　者： ; ; ;

机构地区： 中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室

出　　处： 《生命科学研究》 2002年第4期310-313,共4页

摘　　要： 利用套叠PCR技术将α factor信号肽基因插入pAO815之EcoRⅠ位点,构建成分泌型表达载体pAO815α A.在不改变原克隆位点EcoRⅠ的条件下,借助pAO815之第873位的HindⅢ位点,将pAO815AOX1启动子873 940片段连同α factor信号肽基因序列(246bp)插入pAO815之HindⅢ EcoRⅠ之间,构建成含α factor信号肽基因的分泌型表达载体pAO815α A. The pAO815αA of secrete expression vector was constructed with αfactor signal peptide gene inserted into EcoR Ⅰ site of pAO815 by overlap extension of PCR technique,which 873th940th bps fragment of pAO815 AOX1 promoter with αfactor signal peptide gene was inserted into Hind Ⅲ/EcoR Ⅰ site and a kind of pAO815 containing αfactor signal peptide gene was constructed under nonchanging previous EcoR Ⅰ cloning site.

关 键 词： 套叠 技术 改造 酵母表达载体 分泌型表达载体

领　　域： [生物学]