作　　者： ; ; ; ; ;

机构地区： 湖南农业大学食品科技学院

出　　处： 《现代食品科技》 2015年第5期76-83 162,162,共9页

摘　　要： 研究虎杖内生真菌对虎杖苷转化及转化产物抗氧化活性。采用薄层层析法筛选转化虎杖苷的内生真菌;通过形态学特征和ITS-5.8S r DNA基因序列分析对内生真菌进行鉴定;利用大孔吸附树脂、制备薄层色谱和高效液相色谱分离纯化生物转化产物;以VC、BHT、虎杖苷及5种虎杖苷衍生物为对照,对转化产物清除DPPH·自由基和OH·自由基的生物活性进行比较研究。经筛选发现其中l株内生真菌J3能转化虎杖苷形成转化产物。内生真菌J3 ITS-5.8S r DNA序列全长573bp,Gen Bank登录号KM 434883,确定为烟曲霉Aspergillus fumigatus。转化产物A-2在体外具有清除DPPH·自由基和OH·自由基的能力,且清除OH·自由基的能力高于底物虎杖苷,A-1在体外无清除DPPH·自由基和OH·自由基的能力。虎杖内生真菌Aspergillus fumigatus能对虎杖苷进行生物转化,形成具抗氧化活性的物质。 The microbial transformation of polydatin by endophytic fungi isolated from Polygonum cuspidatum and the antioxidant activity of the transformed products were investigated. Screening of endophytic fungi for microbial transformation of polydatin was carried out using thin-layer chromatography(TLC). Fungi were identified by morphological characteristics and analysis of the ITS-5.8S r DNA region. Polydatin transformation products were isolated and purified by macroporous adsorption resin, preparative TLC, and high-performance liquid chromatography. Using vitamin C(Vc), BHT, polydatin, and five types of polydatin derivatives as standard substances, the DPPH· and OH· radical-scavenging capacities of transformation products were investigated. An endophytic strain J3, capable of transforming polydatin was identified preliminarily as Aspergillus fumigatus(Genbank accession number: KM434883) by morphological characteristics and analysis of the ITS-5.8S r DNA region(573 bp). The transformed product, A-2, showed DPPH· and OH· radical-scavenging capabilities in vitro, while the OH· radical-scavenging ability was higher than that of substrate polydatin. However, transformed product, A-1, did not show DPPH· and OH· radical-scavenging capabilities in vitro. Endophytic fungus Aspergillus fumigatus isolated from Polygonum cuspidatum was thus shown to transform polydatin into products with antioxidant property.

关 键 词： 内生菌 虎杖苷 衍生物 转化 鉴定 抗氧化活性

领　　域： [化学工程]