作　　者： ; ; ; ; ; ;

机构地区： 石河子大学动物科技学院

出　　处： 《中国畜牧兽医》 2014年第12期230-234,共5页

摘　　要： 为研究冷应激对阿勒泰羊脂蛋白脂酶(lipoprotein lipase,LPL)mRNA表达的影响,试验分别采集冷应激前与冷应激后阿勒泰羊6个部位的组织,采用实时荧光定量PCR方法检测各组织中LPL mRNA的表达水平,分析其在不同部位中表达的变化。结果显示,阿勒泰羊LPL mRNA表达量在冷应激后显著或极显著升高(P<0.05;P<0.01),其中颈部肌间脂肪和尾部脂肪冷应激后表达量最高。表明阿勒泰羊在低温下脂肪组织的动员能力较强,使机体产热增加,以抵抗冷应激。 This experiment was to study the effect of cold stress on the lipoprotein lipase(LPL)gene mRNA expression in Altay sheep,tissue samples of six part were collected before and after cold stress,LPL mRNA expression levels were detected using RT-PCR method.The results showed that the LPL mRNA expression of Altay sheep was significantly or extremely significantly increased after cold stress(P<0.05;P<0.01),and the highest expression after cold stress was intramuscular fat of the neck and tail fat,suggested that the Altay sheep's strong mobilization of adipose tissue at low temperatures so that their body heat production increased to resist cold stress.

关 键 词： 冷应激 脂蛋白脂酶 阿勒泰羊

领　　域： [农业科学] [农业科学]