作　　者： ; ; ; ; ; ;

机构地区： 河南理工大学

出　　处： 《光谱学与光谱分析》 2016年第S1期385-386,共2页

摘　　要： 通过原位沉淀法合成了Fe3O4/g-C3N4磁性复合纳米材料,表现出高效的过氧化物模拟酶催化活性,比单一的纳米材料的催化活性强,这归因于Fe3O4磁性纳米粒子与g-C3N4的协同催化效应。本研究以TMB为模型底物,研究了催化条件(温度和pH值)对催化活性的影响。同时结合乙酰胆碱(ATCh)在乙酰胆碱酯酶(AChE)和胆碱氧化酶(CHO)作用下产生H2O2及有机磷农药对AChE的抑制作用,建立了可视化比色法测定有机磷农药残留的简便方法。在最佳条件下,对有机磷农药氧化乐果进行了检测,检测范围为1×10-7~1×10-4 mol·L-1,检测限达到2×10-8 mol·L-1。本方法将在生物传感器构建和环境化学领域具有潜在的应用价值。 Fe3O4/g-C3N4 magnetic nanocomposites prepared in situ precipitation method,which showed the high efficiency catalytic activity of peroxidase mimics,were better than those that the catalytic activity of single nanomaterial.This was ascribed to the synergetic effect that occurred at the interface of Fe3O4 and g-C3N4 nanoparticles.In this study,the effects of the conditions(temperature and pH)on the catalytic activity were investigated with TMB which was used as a model substrate.Meanwhile,a simple and visual colorimetric method was established for determination of organophosphorus pesticide residues,combind with the producation of H2O2 by acetylcholine(ATCh)under the action of acetylcholinesterase(AChE)and choline oxidase(CHO),and the inhibition of AChE by organophosphorus pesticide.Under the best conditions,the detection of organophosphate pesticide omethoatewas implemented in the range of 1×10-7~1×10-4 mol·L-1 and with a detection limit of 2×10-8 mol·L-1.This method will possess potential application value in the field of biosensor fabrication and environmental chemistry.

关 键 词： 有机磷农药 过氧化物模拟酶 氧化乐果

领　　域： [化学工程] [一般工业技术]