机构地区: 中山大学生命科学学院基因工程教育部重点实验室
出 处: 《海洋学报》 2002年第6期99-103,共5页
摘 要: 测定了发生于我国东南海域赤潮原因种Phaeocystissp.及相关种类P globosa和P pouchetii18SrDNA序列,并以Phylip35分析软件构建序列距离矩阵和分子系统发育树图.结果表明,该赤潮原因种与球形棕囊藻P globosa18SrDNA序列完全相同,从分子水平鉴定了该原因种为P globosa.分子系统发育树图表示的P globosa和P pouchetii的分化顺序表明,分布于我国东南海域的P globosa可能是一种本地起源的暖水种.Phaeocystisglobosa不同株系的形态差异,可能是适应不同生活环境的结果.根据分子生物学的数据对有害赤潮藻类进行系统发育分析,并设计用于有害赤潮藻快速鉴定的特异性分子探针,对赤潮的监控和防治具有重要意义. The 18S rRNA genes of 'redtide' related Phaeocystis sp. from the southeast coast of China, and its related species P.globosa and P.pouchetii are sequenced. Phylogenetic trees are constructed by using these 18S rDNA sequences. It is clearly shown that Phaeocystis sp. from the southeast coast of China is remarkably similar to several isolates of P.globosa rather than P.pouchetii. The results demonstrate that the bloomforming species of Phaeocystics most likely originated from an endemic warmwater species. The phenotypes of different members of the genus Phaeocystis are variable, apparently changing in response to environmental conditions.