机构地区: 永州职业技术学院
出 处: 《郴州师范高等专科学校学报》 2002年第5期120-122,共3页
摘 要: 以紫外线为诱变剂,用庆大霉素产生菌JY1-12为出发菌株,选育出性能优良的菌株JY3-5.经正交试验确定其最佳发酵培养基,研究了最佳发酵温度和初始pH值,使其摇瓶效价提高35.1%;30m3灌放大试验,比出发菌株效价提高19.2%. The start strain of JY112 was treated with UVrayirradiation to obtain a strain of micmmonospora purpurea JY35 with high yield gentamich. The culture medium was optimizied for the strain JY35 by L9(34)test, and the effective factors on fermentation was also studied. The fermentation potency of strain JY35 in shaker. the increased by 34.3%, and in 30m3 fermentor its increase was 18.6% as compared with JY112.