作　　者： ; ; (安允权）; (李明娜）; (李沛）; (王顺喜）; (张莹莹）; (陈彦惠）; (吴连成）;

机构地区： 河南农业大学农学院

出　　处： 《分子植物育种》 2016年第8期2113-2120,共8页

摘　　要： 玉米叶面积大小及分布对玉米的有效光吸收、干物质积累和产量的形成有重要作用,探究玉米不同叶位叶面积的遗传机理对高产玉米新品种的选育具有重要意义。本研究以两个叶面积差异显著的自交系为亲本组配了含有259个单株的F2群体;以此群体为作图群体,构建了一张总长1 735.1 c M的遗传连锁图谱,该图谱包含218个SSR标记,平均图距7.96 c M。用复合区间作图法(CIM)对玉米9片叶叶面积(穗上4片叶,穗位叶,穗下4片叶)分别进行了QTL定位分析,共定位到36个叶面积QTL,主要分布于第1、2、3和5染色体上,且控制不同叶位叶面积的QTL有集中分布现象。在第2和5染色体上定位到7个贡献率大于10%的QTL,第2染色体phi090-umc1256标记区间内的3个QTL位点可解释穗上3片叶的表型变异分别为12.7%、13.1%和11.1%;第5染色体umc1563-umc2301标记区间内的4个QTL位点可解释穗位叶及穗下第2、3、4叶的表型变异分别为15.4%、13.1%、12.3%和10.4%;这两个标记区间可能是调控玉米叶面积的重要区段。研究结果有助于进一步探究玉米不同叶位叶面积的遗传机制。 Abs tract The siz e and distribution of leaf area plays a vital role in effective optical absorbability, dry matter accumulation and grain yield in maize. Therefore, the study for genetic architecture on leaf area for different leaf position is significant for breeding maize with new varieties and high yield. In this study, a genetic linkage map covering 1 735.1 c M was constructed based on F2 population, which contained 259 plants derived from a cross between two parent lines which showed a significant difference in leaf area. The genetic map included 218 SSR makers with an average interval of 7.96 c M. QTLs for leaf area of nine leaves in maize(the ear-leaf, the four leaves above the ear and the four leaves below the ear) were identified by composite interval mapping(CIM) and totally36 QTLs were detected. Most of the QTLs were identified on chromosome 1, chromosome 2, chromosome 3 and chromosome 5, in addition, the QTLs of leaf area for different leaf position located in the similar region. Among these 36 QTLs, 7 QTLs explaining more than 10% of the phenotypic variation were identified on chromosome 2and chromosome 5. Among which 3 QTLs between phi090 and umc1256 on chromosome 2 explained 12.7%,13.1% and 11.1% of the phenotypic variation of the 3 leaves above the ear, respectively; 4 QTLs between umc1563 and umc2301 on chromosome 5 explained 15.4%, 13.1%, 12.3% and 10.4% of the phenotypic variation of the ear-leaf and the second, third and fourth leaves below the ear. These two marker interval may be the significant region regulating the leaf area in maize. These results are beneficial to further research for genetic architecture on leaf area for different leaf position in maize.

关 键 词： 玉米 叶面积 标记

领　　域： [农业科学]