作　　者： ; ; ; ; ;

机构地区： 中国食品药品检定研究院

出　　处： 《食品科学》 2017年第6期315-320,共6页

摘　　要： 采用超高效液相色谱-串联质谱法对猪肉中沙丁胺醇、盐酸克伦特罗和莱克多巴胺含量的不确定度进行评估。根据JJF 1135—2005《化学分析测量不确定度评定》和JJF 1059.1—2012《测量不确定度评定与表示》中的有关规定,建立测定猪肉中3种β-受体激动剂残留量不确定度的数学模型,逐层对不确定度来源进行分析。通过对不确定度分量进行量化和合成,得出当猪肉中沙丁胺醇含量为1.99μg/kg时,其扩展不确定度为0.25μg/kg(k=2);当猪肉中盐酸克伦特罗含量为2.04μg/kg时,其扩展不确定度为0.24μg/kg(k=2);当猪肉中莱克多巴胺含量为1.97μg/kg时,其扩展不确定度为0.24μg/kg(k=2)。评定结果表明,影响检测结果的主要因素为标准溶液配制、标准曲线拟合和测量重复性等。 This study aimed to evaluate the uncertainty of measurement in the determination of salbutamol,clenbuterolhydrochloride and ractompaine in pork by ultra performance liquid chromatography-tandem mass spectrometry(UPLCMS/MS).According to JJF1135-2005Evaluation of Uncertainty in Chemical Analysis Measurement and JJF1059.1-2012Evaluation and Expression of Uncertainty in Measurement,a mathematical model was established for uncertainty evaluation.The sources of uncertainty that may be introduced were analyzed and each component of uncertainty was quantified for thecalculation of the combined uncertainty.The results showed that an expanded uncertainty of0.25μg/kg(k=2)was obtainedfor the determination of the presence of1.99μg/kg salbutamol in pork,the expanded uncertainty was0.24μg/kg(k=2)forthe determination of pork samples containing2.04and1.97μg/kg salbutamol.The preparation of standard solution,curvefitting and measurement repeatability were found to be the main sources of uncertainty.

关 键 词： 超高效液相色谱 串联质谱 沙丁胺醇 盐酸克伦特罗 莱克多巴胺 不确定度 猪肉

领　　域： [理学] [理学]