作　　者： ; ; ; ; ; ; (全秋梅）;

机构地区： 广东海洋大学水产学院

出　　处： 《广东海洋大学学报》 2016年第4期17-22,共6页

摘　　要： 采用DNA 条形码技术辅以传统形态分类方法，对雷州半岛马尾藻属（Sargassum）6 常见种进行物种鉴定和系统进化分析。测得6 种马尾藻线粒体COI 基因序列，该序列与GenBank 和BOLD 数据库中马尾藻序列同源性均大于或等于99%，该结果与形态分类学鉴定结果一致。COI 基因序列特征分析表明，6 种马尾藻保守位点421 个，变异位点58 个，简约信息位点21 个，单一突变位点37 个，变异率7.7%，T、C、A、G 平均含量分别为39.1%、18.9%、19.1%、22.9%，A+T 含量（58.2%）高于C+G 含量（41.8%），UUU 所编码的苯丙氨酸（F）使用频率最高，达12.5%，最大似然法估算的转换颠换比值为2.42，种间遗传距离多在0.030±0.013 ~ 0.100±0.016之间。聚类分析结果与形态学鉴定、同源性分析结果一致。 Species identification and phylogenetic relationship in 6 common species of Sargassumcollected from Leizhou Peninsula were analyzed by appling DNA barcoding technology assistedtraditional morphological classification method. Mt-DNA COI gene sequences of 6 species ofSargassum and existing data in GenBank and BOLD database for homology analysis showed that thesimilarity is 99% or more, the molecular data were consistent with the morphological identificationresults. In addition, the analyses on characteristics of COI gene sequence shows that COI gene sequencein 6 species Sargassum including 421 conserved sites, 58 variable sites, 21 Parsim-Informative sites,and 37 Singleton sites, accounted for 7.7%. Nucleartides composition results showed: T, C, A, Gaverage contents were 39.1%, 18.9%, 19.1%, 22.9%, respectively, and A+T content (58.2%) wassignificantly higher than C+G content (41.8%). The highest frequency of amino acid was Phenylalanine(F) which coded by UUU(12.5%). The rate of transition and transversion based on maximum likelihood was 2.42. Interspecific genetic distances were from 0.001 ± 0.001 to 0.113 ± 0.016. The result of clusteranalysis was consistent with that of morphological identification and homology analysis.

关 键 词： 马尾藻属 基因 物种鉴定 分子系统进化 条形码

领　　域： [生物学] [生物学]