作　　者： ; ; ; ; ;

机构地区： 湛江海洋大学农学院

出　　处： 《中国生物防治》 2002年第4期171-175,共5页

摘　　要： 对健康辣椒植株体内的内生细菌及拮抗菌进行了分离筛选。结果表明 ,不同品种、部位及种植地 ,内生细菌的数量有所不同 ,品种间内生细菌的数量变化为 2 83× 1 0 3 ～ 1 346×1 0 4cfu/g(FW) ;各品种以叶片中内生细菌的数量最多 ,其次为根 ,再次为茎 ,果最少 ;不同种植地中 ,前作为水稻田种植辣椒叶片中内生细菌的数量比旱地种植的多 ,而根、茎、果中内生细菌的数量正相反。拮抗、防病测定表明 ,1 0 8株辣椒内生细菌中 ,有 2 8 7%的菌株对香蕉枯萎菌和黄瓜枯萎菌有拮抗作用 ;来自辣椒叶片和茎杆内的BS 2和BS 1两菌株 ,对 1 3种植物病原真菌有较强和稳定的拮抗作用 ,其中对辣椒炭疽病有 57 34%～ 94 0 8%的防病效果 ,并可以在辣椒体内定殖。经鉴定该两菌株为枯草芽孢杆菌。 The endophytic antifungal bacteria were isolated and screened from Capsicum frutescens. Results indicated that population of endophytic bacteria ranged from 2.83×10 3 to 13 46×10 3 cfu per gram of fresh weight although it might vary depending on variety, tissue and planting location of the plant. Population of the endophytic bacteria were biggest in leaf, secondly in root, thirdly in stem and smallest in fruit. Leaf taken from capsicum planted in irrigated field harbored a bigger population of endophytic bacteria than that from non irrigated field. But for the endophytic bacteria in root, stem and fruit, plants growing non irrigated field harbored more than that frowing in irrigated field. Of the 108 strains isolated, 28 7% expressed antagonism against growth of Fusarium oxysporum f. sp. cubense and Fusarium oxysporum f. sp. cucumerinum. Two of them (BS 2 and BS 1) were found not only to be inhibitive to the growth of some fungal pathogens, but also have 57 34%-94 08% control effect to the anthracnose caused by Colletotrichum gloeosporioides. These two strains were identified as Bacillus subtilis.

关 键 词： 分离 辣椒 内生细菌 鉴定 拮抗菌 筛选

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]