作　　者： ; ;

机构地区： 华南师范大学化学与环境学院化学系

出　　处： 《分析化学》 2002年第10期1234-1236,共3页

摘　　要： 在pH 1.1的条件下 ,丁基罗丹明B在 335、380、4 2 0和 4 4 0nm有 4个共振散射峰 ,加入核酸后 ,共振散射峰强度大增 ,其散射光强度与核酸的浓度呈线性关系 ,YRNA、FSDNA、CTDNA的线性方程分别为I =2 .74 +10 .6 4C、I=2 .0 8+16 .19C、I =2 .98+6 .0 8C(mg L) ,基于此建立了具有较高灵敏度、操作简单的核酸测定方法 ,将该方法用于核酸人工合成样品的测定 ,结果令人满意。 At pH 1.1 butyl rhodantine B exhibites four resonance scattering peaks at 335, 380, 420, 440 nm. The intensity was greatly enhanced by the nucleic acids. There were linear relationships between the intensity of resonance scattering and the concentration of the nucleic acids. Linear equations of yeast ribonucleic acid, fish sperm deoxyribonucleic acid, calf thymus deoxyribonucleic acid were I = 2.74 + 10.64 C, I = 2.08 + 16.19 C I = 2.98 + 6.08 C (mg/L) respectively. Based on the above results, a sensitive and simple method was established for the determination of nucleic acids. And six synthetic samples were analyzed satisfactorily.

关 键 词： 共振散射光谱 核酸 丁基罗丹明 染料 测定

领　　域： [生物学] [生物学]